Microfluidic Artificial Organ

 

Principle Investigators: Gilgueng Hwang, Anne-Marie Haghiri-Gosnet

Postdocs: Valeria Lotito

Ph.D students : Julie Lachaux

Recently, advanced in vitro microfluidic cell culture systems have emerged that are capable of replicating the complex three-dimensional architectures of tissues and organs. These microfluidic devices represent valid biological models for investigating the mechanism and function of human tissue structures, as well as studying the onset and progression of diseases, such as cancer.

In this context, in collaboration with P. Couvreur and S. Mura (équipe 7 – Institut Galien Paris Sud), we are developing a tumor-on-a-chip as an innovative 3D in vitro model of pancreatic cancer able to recreate the complex physiology of the tumor microenvironment (PhD of G. Lazzari). The PDMS microfluidic-based platform allows a spatially controlled triple co-culture of Panc-1 tumor spheroids, fibroblasts and endothelial cells (HUVECs) embedded in fibrin-collagen hydrogels. Understanding the efficacy of anticancer drugs on spheroids is the main goal of this project.

Similarly, in collaboration with J-M. Peyrin (UPMC- Jussieu), innovative fluidic devices will be realized for studying neurons growth in confined chambers. Different micro/nanostructures obtained by 3D nanolithography (Nanoscribe system) for forcing neurons pathway (Rapid 3D CNANO and Labex projects). Our objective of this project is to develop new culture experimental model in order to understand the central nervous system.

Finally, in the context of BIOARTLUNG RHU-ANR project (in collaboration with Prof. O. Mercier – Marie - Lannelongue hospital), we are also developing an innovative microfluidic device for blood oxygenation as a durable method of replacing lung function in patients with end-stage, refractory lung disease. This device consists of two networks of channels separated by a porous thin membrane that will ensure gas exchange. An innovative geometry of the blood microvasculature maximizing the gaseous exchange surface is under patenting. Microstructuration processes of blood channels in biocompatible materials as well as nanostructuration of the thin membrane will be improved to get the maximal gas exchange of the system.